![]() DNA content in cell cycle was detected by flow cytometry. Expressions of subunits of telomerase, hTR and hTERT, were assessed by RT-PCR. Morphological phenotype of cultured SHEE cells from the 6th to 30th passages, was examined by phase contrast microscopy, the telomere length was assayed by Southern blot method, and the activity of telomerase was analyzed by telomeric repeat amplification protocol (TRAP). ![]() METHODS: The transgenic cell line of human fetal esophageal epithelium (SHEE) was established with E 6E 7 genes of human papillomavirus (HPV) type 18 in our laboratory. AIM: To search for the biomarker of cellular immortalization, the telomere length, telomerase activity and its subunits in cultured epithelial cells of human fetal esophagus in the process of immortalization.
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